DTT in in vitro transcription Rx

Matt Thomas thomas at molbio.uoregon.edu
Mon Mar 20 19:11:32 EST 1995

In article <li_je-1703951750210001 at cellbio05.med.upenn.edu>,
li_je at a1.mscf.upenn.edu (jie li) wrote:

> Hello,
>   Both in vitro transcription and common PCR rx include 1mM DTT in their
> reaction mix.  I am told that DTT is a reducing reagent, but I still don't
> know its function in that case.  Could someone tell me?
>   Sorry if this is one of the FAQs, but I can not find the FAQ list in
> news.answers.  Where can I get it?
>                                                                         Jie

I think it is to keep the proteins from getting oxidized and dying.  BME also
works in most cases.

Who has time to think of a snappy sig when you are in grad school. :)
Thomas at molbio.uoregon.edu

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