DTT in in vitro transcription Rx
thomas at molbio.uoregon.edu
Mon Mar 20 19:11:32 EST 1995
In article <li_je-1703951750210001 at cellbio05.med.upenn.edu>,
li_je at a1.mscf.upenn.edu (jie li) wrote:
> Both in vitro transcription and common PCR rx include 1mM DTT in their
> reaction mix. I am told that DTT is a reducing reagent, but I still don't
> know its function in that case. Could someone tell me?
> Sorry if this is one of the FAQs, but I can not find the FAQ list in
> news.answers. Where can I get it?
I think it is to keep the proteins from getting oxidized and dying. BME also
works in most cases.
Who has time to think of a snappy sig when you are in grad school. :)
Thomas at molbio.uoregon.edu
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