HELP: Microtubuli or tubulin

Todd DeZwaan DEZWAAN_T at a1.mscf.upenn.edu
Mon Mar 20 21:13:26 EST 1995


In article <3k97ii$lt9 at infosrv.edvz.univie.ac.at>,
michael.micksche at univie.ac.at (Thomas Mohr) wrote:

> Hello,
> 
> I want to examine the influence of some plant alcaloids on the
> assembly of microtubuli. Does anybody know a supply for tubulin ?
> 
> Thanks in advance for your hints !
> 
> Thomas Mohr, University of Vienna

We purify tubulin from bovine brain and use this to polymerize
microtubules in vitro.  The bovine brain can be acquired on ice from your
local slaughter house.  You might try refering to some of Richard Vallee's
work for details regarding this procedure.  Good luck.



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