BAC, cloning, HELP!

SYME,JENNIFER ALBERTA,MS XPC9000 at MUSICB.MCGILL.CA
Mon Mar 20 18:05:54 EST 1995


I have been trying to produce a high molecular weight DNA library using
BAC vectors for some time but have been completely unsuccessful.
I can not get my genomic DNA fragments to ligate to my vector. The
ends of my vector are alright and the ligase seems to work fine.
My problem appears to be with the genomic DNA fragments. Does anyone
have any suggestions that might be able to help me?








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