shake after electroporation?

Michael Cooley szcooley at chip.ucdavis.edu
Mon Mar 20 11:44:05 EST 1995


Qunfeng Dong (qfdong at iastate.edu) wrote:

: Hi,there:

:    I used to shake cells after electroporation at 37C for 1 hrs, somebody
: told me it'd better not shake,just plate cells! the reason is that if cells 
: grow quickly, the plasmid DNA could be kicked out of cells. I never heard
: this before, is it right?

: Qunfeng.

This is probably the silliest thing I have ever heard. Would you think 
that this method is so much different from the CaCl method. It has been 
show much worse for the cells to not allow recovery before subjecting 
them to the antibiotic. 



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