shake after electroporation?
szcooley at chip.ucdavis.edu
Mon Mar 20 11:44:05 EST 1995
Qunfeng Dong (qfdong at iastate.edu) wrote:
: I used to shake cells after electroporation at 37C for 1 hrs, somebody
: told me it'd better not shake,just plate cells! the reason is that if cells
: grow quickly, the plasmid DNA could be kicked out of cells. I never heard
: this before, is it right?
This is probably the silliest thing I have ever heard. Would you think
that this method is so much different from the CaCl method. It has been
show much worse for the cells to not allow recovery before subjecting
them to the antibiotic.
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