Request for actin clone (?)

Tracy Aquilla aquilla at salus.med.uvm.edu
Mon Mar 20 11:11:36 EST 1995


In Article <9503151744.AA08619 at leguin.cc.umanitoba.ca>,
pzhang at cc.UManitoba.CA (pzhang) wrote:
>Hi, netters,
>
>I have two questions.
>
>1. where can I get plasmid DNA (or bugs) for Brassica or Arabidopsis 
>actin gene?
>
>2. Does anybody know the email address of  Dr. Y. Choi at Seoul 
>National University, Republic of Korea?
>
>Thanks in advance.
>
>#######################################################################
>Peijun Zhang                      #                          
>Department of Plant Science       #   Do you love snow?               
>University of Manitoba            #   Come to our Windypeg.
>Winnipeg, MB                      #    
>R3T 2N2                           #       
>Tel:(204) 474-6083                #               
>Fax:(204) 261-5732                #
>E-mail: pzhang at cc.umanitoba.ca    #
>#######################################################################

Find the published sequence, design a pair of oligos, and clone it by PCR or
RT-PCR. This is how I usually obtain the clones I need if the sequence has
already been published. In my experience, this is usually faster and more
reliable than asking someone to send you their clone. Good luck.
    tracy



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