TA cloning -Promega

Mail at scri.sari.ac.uk Mail at scri.sari.ac.uk
Mon Mar 20 13:40:00 EST 1995


In article <3k2tv2$8s0 at mark.ucdavis.edu> ez022056 at rocky.ucdavis.edu (Edward Wang) writes:
>From: ez022056 at rocky.ucdavis.edu (Edward Wang)
>Subject: TA cloning -Promega
>Date: 14 Mar 1995 02:10:42 GMT

>I have been using the TA cloning Kit (p-Gem T system) by Promega.  
>Talking to others, I found out that the T overhangs on the PCR products 
>falls off after a certain amount of time, therefore decreasing the 
>cloning efficiency in a TA system.  The best way was to PCR, and then 
>immediately clone into vector.  Has anyone else heard anyting like this?


>--
>********************************
>Edward H. Wang                 *
>Staff Resch. Assc.             *
>Dept. Internal Med. -Nephrology*
>UC Davis                       *
>********************************

I have the Invitrogen TA cloning kit (pCR vector) and they say to have fresh 
(less than 24 hours old) PCR product for the same reason. I haven't actually 
tried it yet, though.

Nadine Henderson
Scottish Crop Research Institute
Dundee, Scotland

(what's a UCD alumna doing as far away as Scotland?!?)



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