Curing E.coli plasmids

Duncan Clark Duncan at genesys.demon.co.uk
Mon Mar 20 07:30:19 EST 1995


Hi Folks,

Has anyone got a good high efficiency method for curing both colE1 
replicon based plasmids (pBR322 deriv.) and p15A replicon based 
plasmids (pACYC series). EtBr, Acridine Orange, electroporation 
(if plasmids can go in they can also come out!) etc.

Many thanks

Duncan

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