recombinant protein purification

[Dominique Schaller]

Hello Netters,

Sometimes ago somebody published a method to extract recombinant protein overproduced in E. coli.
The method was based on the observation that the overproduced protein is preferentially released
from the cells after some cycles of freezing/unfreezing. I have lost the reference. Could 
somebody help me? 
Thanks in advance.

Dominique Schaller
dschalle at sc2a.unige.ch