An enzyme degrading dsDNA but not ssDNA

John Dixon jpcd0 at mole.bio.cam.ac.uk
Tue Mar 21 09:15:03 EST 1995


In article <3khe3e$aia at mserv1.dl.ac.uk>, jsasse at hgmp.mrc.ac.uk (Mr. J.
Sasse) wrote:

> Hello Netters,
> Does anybody know of an enzyme which will degrade dsDNA randomly
> but not ssDNA. Could I use DNase I with a specialised buffer allowing
digest dsDNA?

How about Exonuclease III (Epicentre Tech)? never used it myself but:-

"Exo III is a 3'to 5' exonuclease which degrades linear duplex DNA
containing 5' overhangs or blunt ends, it is not active on 3'overhangs,
single stranded DNA, or thioester-linked nucleotides..."

Any good?

-- 
John Dixon                     Lab 44 (223) 334131
Wellcome/CRC Institute         Fax 44 (223) 334134
Dept Genetics
Cambridge University    
United Kingdom       e-m: jpcd0 at mole.bio.cam.ac.uk



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