recombinant protein purification

[Mxm]

In article <1995Mar21.095413.25995 at news.unige.ch>, dschalle at sc2a.unige.ch
(Dominique Schaller) wrote:

> Hello Netters,
> 
> Sometimes ago somebody published a method to extract recombinant protein
overproduced in E. coli.
> The method was based on the observation that the overproduced protein is
preferentially released
> from the cells after some cycles of freezing/unfreezing. I have lost the
reference. Could 
> somebody help me? 
> Thanks in advance.
> 
> Dominique Schaller
> dschalle at sc2a.unige.ch
Dear Dominique,
   Bio/Technology Vol 12 Dec 1994 p1357  Johnson and Hecht
  Hope this is what you were looking for.  I was thinking about using this too.
   Peter Emanuel