Direct fluorescein labeling of DNA

Edward Beaty edbeaty at
Tue Mar 21 16:44:38 EST 1995

I'm wondering if it's possible to directly (non-enzymatically) link
fluorescein to DNA.  I know you can incorporate fluoresceinated (?)
nucleic acids into DNA by Klenow or terminal transferase, but I was
wondering if there's a simpler chemical method using fluorescein
isothiocyanate or another deriviative.  Also, if it is possible, has
anyone tried to use this DNA for hybridizations i.e. Southern blots? 
Thanks for your help.

Edward Beaty
Department of Microbiology
University of Illinois-Urbana

Ed Beaty
Ed_Beaty at
"A mime is a wonderful thing to waste."

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