Direct fluorescein labeling of DNA

Edward Beaty edbeaty at uxa.cso.uiuc.edu
Tue Mar 21 16:44:38 EST 1995


I'm wondering if it's possible to directly (non-enzymatically) link
fluorescein to DNA.  I know you can incorporate fluoresceinated (?)
nucleic acids into DNA by Klenow or terminal transferase, but I was
wondering if there's a simpler chemical method using fluorescein
isothiocyanate or another deriviative.  Also, if it is possible, has
anyone tried to use this DNA for hybridizations i.e. Southern blots? 
Thanks for your help.

Edward Beaty
Department of Microbiology
University of Illinois-Urbana

-- 
Ed Beaty
Ed_Beaty at qms1.life.uiuc.edu
"A mime is a wonderful thing to waste."



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