Running Retic lysate on SDS-PAGE

Darren Boehning boehnin1 at jeflin.tju.edu
Tue Mar 21 14:53:54 EST 1995


Dave,
We had the same problem for some time, and solved it by incubating at 45C
for 15 minutes after it is quenched in SDS buffer (as opposed to boiling
the sample before running it out on the gel).  Reason for success with this
method may be that some proteins like to aggregate at boiling temperatures
after quenching, and therefore this is eliminated by the lower incubation
temperature.  

Darren Boehning
Thomas Jefferson University
boehnin1 at jeflin.tju.edu



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