Soeren Jensby Nielsen sjn at
Wed Mar 22 02:39:43 EST 1995

Hi netters 

I am working with a human gene that is expressed in all tissues except skeletal
muscle. I would like to examine why my gene is extinguished in skeletal muscle
using in vitro reporter gene assays (like CAT etc.) Now the question is how
do I do this? In other words : Which cell line meets my demands of being
of terminally differentiated striated muscle origin and how do I treat it?

All suggestions are extremely welcome


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