PCRs no longer working. HELP!

Dr Keith Grimaldi regokag at ucl.ac.uk
Wed Mar 22 13:16:09 EST 1995

>>On Thu, 16 Mar 1995, John Compton wrote:
>>> I have been working for 1 1/2 years on a project involving mapping and,
>>> now, sequencing of blood and buccal DNA samples from patients.  Initially,
>>> I experienced no problems amplifying these DNAs for microsatellite
>>> analysis and for template synthesis prior to sequencing.  Now, however, a
>>> dark cloud has appeared and only  specific, primarily buccal, DNAs will
>>> amplify. 

You could try DMSO (1-10%) of formamide (1-10%) in the PCR mix. I have found
it useful sometimes.
Keith Grimaldi, Dept. Oncology
                University College London   TEL: (0)171-636-8333  ext:3419
                91, Ridinghouse Street      FAX: (0)171-436-2956 
                LONDON, W1P 8BT, UK.            

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