TA cloning kit (In Vitrogen)

[Wayne Gottlieb]

In article <3kmdh1$512 at news.univ-rennes1.fr>, Onno at univ-rennes1.fr
(Onno_Myriam) wrote:

> I have been using the TA cloning kit by In Vitrogen.
> Recently, the compagny has sent the vector resuspended in TE buffer. 
> I found that the cloning efficiency has decreased. A lot of  white colonies do not contain insert !
> Has anyone the same problem ?
> 
> 
> Yes, I once tried to TA clone PCR products for site directed mutagenisis and found 50% to be revertants (white, but containing no insert)