Msp1 Genomic Digests

Duncan Clark Duncan at genesys.demon.co.uk
Wed Mar 22 12:13:43 EST 1995


In article: <3kpb0e$d5e at mserv1.dl.ac.uk>  <DYDM6025 at iruccvax.ucc.ie> writes:
> 
> 
> Having trouble digesting genomic DNA with Msp1, using Maniatis
> protocol.
> DNA is concentrated 1ug/ul, digesting 6ug O/N with 4 fold excess RE
> 
> High molecular material still remains.
> 

Methylation could give you this problem. If so there is no solution. 
Alternatively try adding spermidine I think tio 0.5mM final but don't quote
me on that.

Duncan


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