Optimum temp for PCR primer annealing

Andy Law Big Nose Andy.Law at bbsrc.ac.uk
Wed Mar 22 12:14:15 EST 1995

In article <22MAR95.10855568.0089 at SEMOVM.SEMO.EDU>, Allen Gathman
<C741SCB at SEMOVM.SEMO.EDU> wrote:

  > Does anyone have an algorithm for calculating the optimum temperature to
  > use for annealing PCR primers, based on sequence and length?  I know
  > that many commercial programs will do this, but we don't have one.  Of
  > course, if anyone knows where I can get a shareware program that does
  > primer design, I'd be interested in that too.

Primer (version 0.5) can be had from the Whitehead Institute.

ftp to genome.wi.mit.edu in directory /distribution/prime.0.5
                     ( I think...)
Andy Law

( Andy.Law @ bbsrc.ac.uk )
( Big Nose in Edinburgh )

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