Church Buffer, high background

DYDM6025 at DYDM6025 at
Wed Mar 22 09:13:48 EST 1995

Having trouble with hybridisations for S.Blot, hybridisations are being
carried out in plastic tubs.

O/N hybridisations results in evaporation of the buffer, membrane sticks to the
Result of which is very high background.
Can volume of buffer be increased without a decrease in the sensitivity ?.

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