Pischia expression

[H. David Fischer]

Jonathan Kurtis <Jonathan_Kurtis at Brown.edu> writes:
 
>The Pischia pastoris expression system from Invitrogen contains vectors
>which do not add a His tag to the recombinant protein. Is there a good
>reason for this. Has  anyone added a His tag and used it for purification
>from this system. Thanks,
 
In the March issue of BIO/TECHNOLOGY there is a paper by Ridder et al. They
expressed a secreted single-chain Fv rabbit antibody with a HIS tag using
the Invitrogen Pichia exp expression kit.