peptide expression in E.coli

M.R.douglas at rheuma.bham.ac.uk M.R.douglas at rheuma.bham.ac.uk
Wed Mar 22 04:54:08 EST 1995


I am interested in producing peptides (10-30 a.a. in length) in E.coli with a
view to labelling them isotopically for NMR experiments.  Does anyone out
there have any hints, tips or references as to the best way to go about this?
And which is the best purification method for sorting out the good from the bad?
Getting them synthesised in the old fashioned way would break the bank, I'm
afraid.

Here's hoping!

Mike.



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