M13 antibodies for phage display
williams at bscr.uga.edu
williams at bscr.uga.edu
Wed Mar 22 16:54:36 EST 1995
In article <2F6DDD41 at smtpgw.lifetech.com>, DSCHUSTE at lifetech.com ("Schuster, David") writes:
>
>Dear Netters,
>
>I am looking for antibodies directed against M13 coat proteins to use in
>phage display work. I want to set up an ELISA assay to verify that my
>cloned insert is indeed expressed on the phage surface. Does anyone know
>of sources, commercial or academic, for such reagents?
>
>Please reply directly to my E-mail address (dschuste at lifetech.com).
>
>Thanks in advance.
Five-prime three-prime sells sheep anti-M13 polyclonal serum, either in kits
or as individual reagents. They are located in Boulder CO; phone:
800-533-5703.
Have had no difficulty in using it, but M13 phage are sticky and give
high background in most assays.
Good luck.
Myron
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