protein marker for insect microsomes

[Darren Fast]

Hi There.

Does anyone out there have experience with subcellular fractionation of
Sf-9 or Sf-21 cells (commonly used in the baculovirus protein expression
system)?  Any ideas for marker enzymes, or proteins would be greatly
appreciated.  I am most interested in obtaining a microsomal fraction, but
markers for other fractions i.e., nuclei, mitochondria would help greatly.


Thanks in advance,

Darren