protein marker for insect microsomes

Darren Fast fast at biochem.wisc.edu
Wed Mar 22 22:29:14 EST 1995


Hi There.

Does anyone out there have experience with subcellular fractionation of
Sf-9 or Sf-21 cells (commonly used in the baculovirus protein expression
system)?  Any ideas for marker enzymes, or proteins would be greatly
appreciated.  I am most interested in obtaining a microsomal fraction, but
markers for other fractions i.e., nuclei, mitochondria would help greatly.


Thanks in advance,

Darren



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