GFP in CHO cells
grtfulned at aol.com
Wed Mar 22 20:37:37 EST 1995
Chris, we have not had success in getting a signal from GFP in 293 cells
(human cells) transfected with GFP expression vector using a FACS assay.
We know our transfections work with high efficiency since a control vector
that makes a cell surface protein is easily detetable by staining with
FITC-lalbelled antibody. Is there some trick to detecting GFP? Does it
work in any cell? Do we need a special FACS machine? Any tips, please
let me know. Ned Landau, NYU Medical School, Dept. of Pathology. Can
also send message to ned at phri.nyu.edu. Thanks.
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