M13 ssDNA degradation?
dubear at offroad
Thu Mar 23 10:30:25 EST 1995
I was wondering if someone had any insight to a problem we have
encountered. After isolating ssDNA from M13 (by a variety of methods)
we find that if the DNA is resuspended in water and heated to 90 C for
10 min., the DNA appears to degrade. This doesn't occur when not
heated or when resuspended in TE (heated or not). Does anyone have
any insights on this? You can reply directly by e-mail and I will
summarize the respones.
dubear at whitewater.chem.wisc.edu
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