GFP problems. Any tips?

Grtful ned grtfulned at
Thu Mar 23 09:31:53 EST 1995

We have been unable to see green fluoresecnt protein by FACS or by UV
microscopy after transfecting 293 cells.  The problem is not the
transfection, since control transfection of a cell surface protein vector
detected with FITC-labeled antibody worked great.  Is there some trick to
detecting it?  Is it possible that it doesnt work in all cell types?  Does
anyone have a control cell line to transfect or any positive controls that
might help us to figure out this problem?  Thanks. 

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