Sherif Abouelela sherif at ORCHID.UCSC.EDU
Thu Mar 23 22:17:46 EST 1995

Hi to all:

	Does anybody know an easy way to reduce non specific binding of
proteins to the Ni-NTA spin column. I am trying to purify a 54,000
dalton  protein from yeast using a six his tag. While I can see my protein
using immunoblotting tons of other stuff also stick to the column [as judge by
staining]. Any ideas or tricks.

Sherif Abouelela

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