recombinant protein purification
dschalle at sc2a.unige.ch
Thu Mar 23 11:22:26 EST 1995
In article <pemanuel-2103951618360001 at beaker.ab.umd.edu>, pemanuel at umabnet.ab.edu (Mxm) says:
>In article <1995Mar21.095413.25995 at news.unige.ch>, dschalle at sc2a.unige.ch
>(Dominique Schaller) wrote:
>> Hello Netters,
>> Sometimes ago somebody published a method to extract recombinant protein
>overproduced in E. coli.
>> The method was based on the observation that the overproduced protein is
>> from the cells after some cycles of freezing/unfreezing. I have lost the
>> somebody help me?
>> Thanks in advance.
>> Dominique Schaller
>> dschalle at sc2a.unige.ch
> Bio/Technology Vol 12 Dec 1994 p1357 Johnson and Hecht
> Hope this is what you were looking for. I was thinking about using this too.
> Peter Emanuel
Thanks to the people who send me the reference.
I have tried the method yesterday and surprisingly it worked fine for my
proteins (20 kDa, soluble, in hms174).
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