recombinant protein purification

Dominique Schaller dschalle at sc2a.unige.ch
Thu Mar 23 11:22:26 EST 1995


In article <pemanuel-2103951618360001 at beaker.ab.umd.edu>, pemanuel at umabnet.ab.edu (Mxm) says:
>
>In article <1995Mar21.095413.25995 at news.unige.ch>, dschalle at sc2a.unige.ch
>(Dominique Schaller) wrote:
>
>> Hello Netters,
>> 
>> Sometimes ago somebody published a method to extract recombinant protein
>overproduced in E. coli.
>> The method was based on the observation that the overproduced protein is
>preferentially released
>> from the cells after some cycles of freezing/unfreezing. I have lost the
>reference. Could 
>> somebody help me? 
>> Thanks in advance.
>> 
>> Dominique Schaller
>> dschalle at sc2a.unige.ch
>Dear Dominique,
>   Bio/Technology Vol 12 Dec 1994 p1357  Johnson and Hecht
>  Hope this is what you were looking for.  I was thinking about using this too.
>   Peter Emanuel
Thanks to the people who send me the reference.
I have tried the method yesterday and surprisingly it worked fine for my
proteins (20 kDa, soluble, in hms174).

Dominique Schaller



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