PCR amplification of cloned insert

Gil kgilbert at cmp.hmc.psu.edu
Thu Mar 23 11:49:07 EST 1995


Greetings,

I have a 2kb insert cloned into Bluescript and access to a
thermocycler. I would like to PCR amplify this insert, but have no PCR
experience.  The vector has T3 and T7 promoters and insert is arranged
with T7 transcription for antisense.  No sequence data is available. 
Could someone please inform me of reaction conditions and cycle
profiles to PCR amplify this insert. 

Thank you in advance. 

KA Gilbert
Dept. Cell and Mol Physiol
Penn State Univ, Col of Med
Hershey, PA 17033
Voice: 717-531-8997
FAX:   717-531-7667
e-mail: kgilbert at cmp.hmc.psu.edu



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