Transiet transfection of neurons

[Matthew Frosch]

Does anyone have experience with transient transfection of primary
cultures of neurons?  I am interested in several questions:

      What types of cultures have you used?

      What did you use to transfect (CaPO; DEAE; Liposomes)?

      What promoter did you use as a control (normalization) 
              construct?

Any advice or direction would be appreciated.

Matthew Frosch

frosch at bics.bwh.harvard.edu