Subcloning large fragments

Nick Ellison agpnwe at pnv.palm.cri.nz
Thu Mar 23 03:07:24 EST 1995


I need some advice on subcloning large fragments (15-20kb) from a lambda
vector into a plasmid vector.  Is it possible to reliably subclone such
fragments?  If so, what host/plasmid combinations are recommended?

Nick Ellison



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