Optimum temp for PCR primer annealing

Hui Yuk Fun wayhui at hkusub
Fri Mar 24 05:57:56 EST 1995

There is a function called 'prime' in GCG version 8, it selects primers
for the sequence you specified. This function calculates the annealing
temperature based on two papers appeared in Nucleic Acids Res. I used
the same method to calculate the annealing temp for my PCR, and the
result is unexpectedly good. You can get the two reference title in the
HELP menu of GCG version 8.

Wayland Yuk-Fun HUI, MPhil 
Department of Paediatrics,
Faculty of Medicine, 
The University of Hong Kong.

Pager    : 1128631 A/C 7260
E_mail   : 1) wayhui at hkusua.hku.hk
           2) wayhui at hkucc.hku.hk
Telephone: (852)-2855-4898
Fax      : (852)-2855-1523

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