Subcloning large fragments
susini at ceph.cephb.fr
Fri Mar 24 05:16:42 EST 1995
about subcloning large fragments of DNA :
- did you already used other strans than XL1 blue !
- do you transform your cells by electroporation or heat shock ?
- how don you prepare your cells for transformation ?
I have many answers for those who are interested in transformation,
but I have many problems with the low efficacity of my transformation
I think that my cells are not well prepared
I hope you`ll understand my english
More information about the Methods