ugha103 at qmw.ac.uk
Fri Mar 24 05:28:46 EST 1995
kferri at email.unc.edu (Karen Ferri) wrote:
> Hello! Please pardon the simple question, but I just started my first
> job as tech. and therefore haven't learned everything yet.
> Homogenized some tissue, isolated and purified the RNA, checked OD's, and
> ran on a gel to check RNA integrity. For 1 sample, the gel lane was
> blank (not even smeared, which would indicate denaturation). I ran 3
> gels to be sure the problem of possible RNA omission to gel lane was
> addressed. Still no run, even after adding extra RNA to lane. The  of
> the sample (I checked it twice) was 17% lower the second time, but acc.
> to my boss, still not low enough to not show up.
> Any ideas? Thanks in advance!
More information about the Methods