Randall J. Cohrs (COHRS-R at Oberon.HSC.Colorado.edu) wrote: : Does anyone know of a restriction endonuclease with a non-palindromic : recognition site ? : thanks If you can find a list of enzymes from a supplier you will find that there are many REs that recognize non-palindromic sites. eg Bgl1, BsaM1, Bsc91I, Bst71I, I-PpoI to name a few. Jill