Problems with densitometry!....for WESTERN BLOTS

Gerard J Madlambayan gjmadlam at UMICH.EDU
Fri Mar 24 12:43:15 EST 1995

On Wed, 22 Mar 1995 jfs at wrote:

> Gerard and netters worldwide,
> From what I understand, the reason your short exposures
> are darker than than your longer exposure is your emmission
> time course.  Check the info that accompanies the reagent.
> If you do the short exposure during the steep part part of 
> emmission time course curve and the long exposure later, it 
> would explain the effect you discribed.  I hope that made
> sense.
> Jean jfs at
> from Miles (soon to Bayer) Research Center

Hi Jean,
Thanks for the info.  I see what you mean.


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