Problems with densitometry!....for WESTERN BLOTS
Gerard J Madlambayan
gjmadlam at UMICH.EDU
Fri Mar 24 12:43:15 EST 1995
On Wed, 22 Mar 1995 jfs at wh.bayer.com wrote:
> Gerard and netters worldwide,
> From what I understand, the reason your short exposures
> are darker than than your longer exposure is your emmission
> time course. Check the info that accompanies the reagent.
> If you do the short exposure during the steep part part of
> emmission time course curve and the long exposure later, it
> would explain the effect you discribed. I hope that made
> Jean jfs at mrc.com
> from Miles (soon to Bayer) Research Center
Thanks for the info. I see what you mean.
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