Problems with densitometry!....for WESTERN BLOTS

[Gerard J Madlambayan]

On Wed, 22 Mar 1995 jfs at wh.bayer.com wrote:

> Gerard and netters worldwide,
> From what I understand, the reason your short exposures
> are darker than than your longer exposure is your emmission
> time course.  Check the info that accompanies the reagent.
> If you do the short exposure during the steep part part of 
> emmission time course curve and the long exposure later, it 
> would explain the effect you discribed.  I hope that made
> sense.
> Jean jfs at mrc.com
> from Miles (soon to Bayer) Research Center
> 

Hi Jean,
Thanks for the info.  I see what you mean.

Gerard