cosmid subcloning

William Jia wjia at
Fri Mar 24 20:37:03 EST 1995

Hi, netters,
    We recently started to clone a gene into a cosmid which is about 30kb.
Our insert is 8kb. It is going to insert into Xba/Bgl II sites. However, in
our first two trials, we have got no colony at all (in DH5a). I wonder if
there is anybody who are familiar with cosmid subcloning may have any
suggestion. We know that circular cosmids transformation itself was OK. Any
special tricks for cosmid ligation?
    Thanks in abvance.


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