Transfections using QIAGEN
Martin D. Leach
leach at bu.edu
Sat Mar 25 10:06:16 EST 1995
Just my 2cents...
I had problems with my transfections in a very sensitive cell-culture
system..so in my maxipreps I applied 200ml instead of 500ml per qiagen 500
column and the transfections gave good results.
I assume I was overloading the column with protein junk and some got
eluted and screwed the transfection up..
(no affiliation with qiagen)
..... Martin Leach Email:leach at bu.edu
_|____ Dept. of Pharmacology Phone: (617) 638-5323
/ o / Boston Univ. School of Med. Fax: (617) 638-4329
_/ |-/__==/ 80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118 "Not the old underpants
USA on your
My homepage: http://220.127.116.11/Leach.html
Biotech company listing: http://18.104.22.168/jumper/biolcomm.html
p.s. try BioMOO -virtual biology on the internet..
telnet bioinfo.weizmann.ac.il 8888
More information about the Methods