Stratagene's Quik Hybridization solution

ellenmq at ucs.indiana.edu
Sat Mar 25 16:03:49 EST 1995


Jun IKEDA (jikeda at hsp1.hsp.co.jp) wrote:
: books at mcvax2.d48.lilly.com wrote:
: >
: > Has anyone had experience using Stratagene's Quik Hybridization solution 
: > for Northerns? Is the hybrid time realy shorteed to 1-2 hours. 
: > Thanks DPSMITH
: > internet:smith_dennis_p at lilly.com

Yes, I've used it for 2 years with beautiful results, 15 min prehyb, 
1 hour hyb, 2X 30 min RT washes, with an optional higher stringency at 65C.
Autorad time with intensifying screen was between 2-12 hours.

Ellen Quardokus
ellenmq at bronze.ucs.indiana.edu



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