SURE vs. NovaBlue competent cells

Malcolm Campbell malcolm at unity.ncsu.edu
Mon Mar 27 11:08:33 EST 1995


A little while back someone posted that Strategene's SURE cells harbour
a Tn5 transposon.  Has anyone had any problems with these cells?  

We have been having problems with what looks like homologous recombination
events with pBIN-based plasmids and with another group of plasmids which
have an insert repeat.  To date we have been using Novagen's Novablue
competent cells.  Has anyone has any problems with these cells?  

Our transformation efficiency is not really a problem.  It's just that we
appear to be losing "chunks" between sequences that are repeated in the
plasmids.  The NovaBlue cells are supposed to be recA1 mutants so I'm at
a quandry as to what is going on.  My druthers at present are to switch to
a massively recombination defficient strain like SURE; hoping that that
may solve the problem.  Problem is I don't know anyone with experience
with SURE cells.

Any advice for what should be routine subcloning experiments most appreciated.

Best regards,

Malcolm

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+ Malcolm M.Campbell                  + malcolm at unity.ncsu.edu      +
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