"Mayer, Jorge ", 3637
J.MAYER at CGNET.COM
Mon Mar 27 10:56:22 EST 1995
In article <3l5203$2js at ralph.vnet.net>, icagen at vnet.net (Chris Silvia)
> In article <baxley-2603951454190001 at fp1-molbio-13.uoregon.edu>,
baxley at molbio.uoregon.edu (Lara Endres Baxley) says:
> >Does anyone know what Nonidet P-40 does and where I can get some? A
> >protein purification protocol calls for 0.01% Nonidet P-40 in all of the
> Try Calbiochem. Sigma doesn't carry this any more.
>Sorry Chris but Sigma still carries it (1995 catalogue, cat. number 6507 -
usual disclaimer). And to Laura, NP-40 is a nonionic detergent; I use it
to remove virus envelope and permabilize virus capsid.
kfischer at gpu.srv.ualberta.ca
tyr-2 at bones.biochem.ualberta.ca
Nonidet P-40 is the same as Triton X-100, which is a common nonionic
detergent used in many extraction protocols to solubilize membranes, very
good for nuclear membranes. As already somebody stated, it has a polar
polyethylenglycol chain and a non-polar tail to interact with membranes,
leading to the formation of lipids trapped in water-soluble micelles.
-Jorge <j.mayer at cgnet.com>
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