An enzyme degrading dsDNA but not ssDNA

Paul Robert Fisher fisher at lumi.latrobe.edu.au
Mon Mar 20 04:41:22 EST 1995


jsasse at hgmp.mrc.ac.uk (Mr. J. Sasse) wrote:
>
> 
> 
> Hello Netters,
> Does anybody know of an enzyme which will degrade dsDNA randomly
> but not ssDNA. Could I use DNase I with a specialised buffer allowing digest dsDNA?
> Thanks a lot for all you help.
> Jurgen
> 
> Jurgen Sasse
> email: js1 at mole.bio.cam.ac.uk

Any restriction enzyme will be specific for dsDNA. If you used
a mixture of frequent cutters you could get close to what you want
perhaps.

Paul Fisher



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