in vitro transcription/translation
abrick at mailhost.tcs.tulane.edu
Mon Mar 27 12:45:11 EST 1995
On Sat, 25 Mar 1995, Yasha Hartberg wrote:
> I am looking for advice on finding a good in vitro
> transcription/translation system for producing protein from a cDNA clone.
> Any suggestions would be greatly appreciated.
I did direct comparisons of in vitro translation kits a few years ago.
Promega's nuclease treated Rabbit Reticulocyte Lysate consistently
outperformed (based % incorporation) equivalent kits from NEN, Ambion,
Promega's TnT coupled Reticulocyte Lysate Systems consistently gave
4-fold higher percent incorporation than even Promega's standard
translation kit. In my opinion, it's the best available.
Brick Ayola, Ph.D.
Tulane School of Medicine
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