in vitro transcription/translation

Brick Ayola abrick at
Mon Mar 27 12:45:11 EST 1995

On Sat, 25 Mar 1995, Yasha Hartberg wrote:

> I am looking for advice on finding a good in vitro
> transcription/translation system for producing protein from a cDNA clone. 
> Any suggestions would be greatly appreciated.

I did direct comparisons of in vitro translation kits a few years ago. 
Promega's nuclease treated Rabbit Reticulocyte Lysate consistently 
outperformed (based % incorporation) equivalent kits from NEN, Ambion, 
and BRL.

Promega's TnT coupled Reticulocyte Lysate Systems consistently gave 
4-fold higher percent incorporation than even Promega's standard 
translation kit. In my opinion, it's the best available.


Brick Ayola, Ph.D.
Tulane School of Medicine

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