degenerate primers PCR

[David B. Stern]

Does anyone have some tips on using degenerate primers to PCR genomic and
cDNA libraries.
I wonder how many different parameters to vary, etc.  My primers were made
against a degenerate yeast  sequence and will be used on plant DNA.  Some
of them are apt to form primer-dimers, etc. and misprime  (especially at
the lower temps. I will need for initial cycyles)  any suggestions on how
to get what I want?

Thanks
Phil Kogan
phk1 at cornell.edu