degenerate primers PCR

David B. Stern ds28 at cornell.edu
Tue Mar 28 13:05:40 EST 1995


Does anyone have some tips on using degenerate primers to PCR genomic and
cDNA libraries.
I wonder how many different parameters to vary, etc.  My primers were made
against a degenerate yeast  sequence and will be used on plant DNA.  Some
of them are apt to form primer-dimers, etc. and misprime  (especially at
the lower temps. I will need for initial cycyles)  any suggestions on how
to get what I want?

Thanks
Phil Kogan
phk1 at cornell.edu



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