LOW EFFICIENCY OF PCR CLONING AND FASLE WHITE COLONIES

Xin-Hua Zhou xz at CHRISTA.UNH.EDU
Tue Mar 28 11:41:54 EST 1995


Hi, Netter:
I am using Invitrogen TA cloning kit to clone my PCR products (400-500bp) 
with very low efficency (2%). PCR and restriction analysis did not 
show expected bands when I pick up white colonies to isolate plasmids. I 
would like to have your suggestions on this problem.
 Thank you in advance!

  Zhou, UNH



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