unexplained insert from PCR clone?

Steve Bryson steve at gene4d.med.utoronto.ca
Tue Mar 28 10:39:59 EST 1995

I've cloned a gene from a human cDNA library by PCR. The sequence 
indicates a 14 b.p. insert relative to the published sequence.
Consequently, a stop codon is introduced immeadiately following the
insertion. Any ideas as to the source of this insertion mutation?

Steve Bryson 
Dept. of Biochemistry
University of Toronto
 E-mail: steve at gene4d.med.utoronto.ca

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