in vitro transcription/translation
Manuel Maria SIMON
Simon at mail.boku.ac.at
Tue Mar 28 11:07:11 EST 1995
Yasha at bigraf.tamu.edu (Yasha Hartberg) wrote:
> I am looking for advice on finding a good in vitro
> transcription/translation system for producing protein from a cDNA clone.
> Any suggestions would be greatly appreciated.
I used PROMEGA coupled Reticulocyte Lysate. It´s called TNT and does
transcription and translation in one tube.
It worked really fine (To come first: I´m NOT from PROMEGA!). Since
it is not very cheap we also tried to rebuild the kit which also
worked. To my opinion to optimize your kit yourself pays only
if you plan to translate frequently for a long period of time.
Manuel M SIMON PhD
Center of Applied Genetics
Univ. BOKU Vienna
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