cDNA library construction

A van der Plas PLAS at rullf2.LeidenUniv.nl
Tue Mar 28 09:35:53 EST 1995


Dear netters, standard construction of a cDNA library requires a lot of
total RNA (3-6 mg) or 10 x 9 cells. Because we have a limited amount of cells
(max 10 x 5 cells), we are looking for a good protocol/strategy to obtain
a (PCR amplified) representative, full length library. Did anyone used
Clontech UniAmp kit and what was your experience with this kit.
Any suggestions are wellcome.
Arie van der Plas
Dept. of Cellbiology
Univ of Leiden



More information about the Methods mailing list