RNA from Spleen - Help!
aredwood at uniwa.uwa.edu.au
Tue Mar 28 03:18:24 EST 1995
I had trouble with spleen RNA with the brain working perfectly in mice.
I don't think I ever fixed it that well however it seemed to work
better if I didn't use too much tissue at the start. ie less spleen
better result. I also found alot of DNA contamination when I used too
much spleen tissue.
Aaron Pawlyk (aaron at ccat.sas.upenn.edu)
wrote: : Oh. One more thing on the spleen RNA isolation.
: There isn't any RNAse contamination coming from equipment, tubes or
: solutions. I routinely do spleen isolation side by side with brain
: isolations using the same reagents, equipment, protocol and the RNA
: isolated from the brain is fine.
: Aaron Pawlyk
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