Invitrogen SP6 and T7 primers...

E. J. Meharra em35+ at andrew.cmu.edu
Wed Mar 29 20:36:25 EST 1995


Invitrogen sells SP6 and T7 primers that can be used for sequencing
inserts in their pCDNAI vector. These are 19 and 20 mers, respectively.
Has anyone designed and used larger primers (25mers+) with this vector
for PCR?  Information regarding successful or unsuccessful attempts
would be appreciated. BTW, I talked to Invitrogen but they could not be
of any help.

Thanks,
John



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