PCRs no longer working. HELP!

Jim Gore jag at qucdn.queensu.ca
Thu Mar 30 20:46:17 EST 1995


In article <Pine.SUN.3.91.950329111616.20309A-100000 at pip>, Dawn Gray <gray at oci.utoronto.ca> says:
>
>Have you checked/calibrated the PCR machine to make sure it is still ramping
>correctly?

Good suggestion, Dawn.  A couple of years ago we were having problems with our
PCRs using a Perkin-Elmer Thermocycler.  It turned out the problem was that 
the level of antifreeze (didn't know they contained that) was down.  Consequently,
the machine wasn't working properly.  Here's hoping the problem in this case is
as simple.

Jim



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